|
KMID : 1004820130140030160
|
|
Journal of Biomedical Research
2013 Volume.14 No. 3 p.160 ~ p.164
|
|
|
A sensitive and specific polymerase chain reaction to detect Mycoplasma hyopnemoniae using Mycoplasma protein P97 gene
|
|
Hong Sun-Hwa
Park Sang-Ho
Lee Yun-Seong
Kim Ok-Jin
|
|
|
Abstract
|
|
|
|
Mycoplasma (M.) hyopneumoniae is the causative agentof swine enzootic pneumonia, a disease that is prevalent inevery country where pigs are raised. In this study, we aimedto develop a sensitive and specific PCR assay to detect M.hyopneumoniae in pigs. The suitability of this PCR assayfor the detection of mycoplasmal infection was also testedusing clinical lung samples from slaughtered pigs. We developeda probe and M. hyopneumoniae-specific primerpairs, MhyoP-F and MhyoP-R, for the new PCR assaybased on regions in the Mycoplasma protein P97 gene thatare unique to M. hyopneumoniae. The developed PCR assaywas very specific and sensitive for the detection of M.hyopneumoniae. The assay was able to detect the equivalentof 10 pg of target template DNA, which indicates that theassay was very sensitive. In addition, the M. hyopneumoniaePCR assay detected only M. hyopneumoniae and no otherMycoplasma spp. or bacterial species of another genera.Further, the newly developed PCR assay effectively detectedM. hyopneumoniae infection in pigs. We suggest that thisPCR assay using M. hyopneumoniae-specific primer pairs,MhyoP-F and MhyoP-R, will be useful and effective formonitoring M. hyopneumoniae infection in pigs.
|
|
|
KEYWORD
|
|
|
Mycoplasma, M. hyopnemoniae, Mycoplasma protein P97, PCR, diagnosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|